Thank you, Ray, and good afternoon, everyone. Before I begin my prepared remarks, I'd like to call your attention to our third quarter earnings press release that was issued earlier this morning. If you need a copy, it can be accessed on our website at ashs.com at Press Releases under the Investors tab.
Now turning to our third quarter results. For the 3 months ended September 30, 2021, revenue decreased 12.2% to $4.099 million compared to revenue of $4.670 million for the third quarter of 2020. Third quarter revenue for the company's proton therapy system installed at Orlando Health in Florida decreased 23.4% to $1.293 million compared to revenue for the third quarter of 2020 of $1.687 million. Total proton therapy fractions in the third quarter were 973, a decrease of 40.4% compared to 1,632 proton therapy fractions in the third quarter of 2020.
Revenue for the company's Gamma Knife operations decreased 5.9% to $2.806 million for the third quarter of 2021 compared to $2.983 million for the third quarter of 2020. Gamma Knife procedures decreased by 10.9% to 336 for the third quarter of 2021 from 377 in the same period of the prior year.
Gross margin for the third quarter of 2021 increased 28.9% to $1.467 million or 35.8% of revenue compared to gross margin of $1.138 million or 24.4% of revenue for the third quarter of 2020.
Selling and administrative costs decreased by 1.4% to $1.119 million for the 3-month period compared to $1.135 million for the same period in the prior year due to lower legal accounting and tax fees. Interest expense decreased 36.2% to $162,000 compared to $254,000 for the same period in the prior year.
Operating income for the third quarter of 2021 was $186,000 compared to an operating loss of $251,000 in the third quarter of 2020, a positive swing of $437,000. Net income in the third quarter was $33,000 or $0.01 per diluted share compared to a net loss of $209,000 or $0.03 per diluted share for the third quarter of 2020.
Fully diluted weighted average common shares outstanding were 6.123 million or 6.049 million for the third quarter of 2021 and 2020, respectively. Adjusted EBITDA, a non-GAAP financial measure, was $1.562 million for the third quarter of 2021 compared to $1.979 million for the third quarter of 2020.
At September 30, 2021, cash, cash equivalents and restricted cash was $7.168 million compared to $4.325 million at December 31, 2020. Shareholders' equity at September 30, 2021, was $23.953 million or $4.08 per outstanding share. This compares to shareholders' equity at December 31, 2020, of $23.650 million or $4.08 per outstanding share.
I'll now turn the call over to Craig for the third quarter operational review. Craig?